Nagoya Journal of Medical Science

A series of transplant-explant experiments using the H 4-II-E rat hepatoma cell line and golden hamsters have been carried out. The original tissue culture cells. which had a modal chromosome number of 51, implanted into the cheek pouches of irradiated hamster (400 rads) produced tumors which were typical hepatomas histologically. Cells from these tumors were placed back into tissue culture and were transferred twice more into the cheek pouches of irradiated animals and tissue culture. These were termed the first. second and third selection, respectively.
At each stage of selection. chromosome counts, karyotypes and histological and biological characteristics of the cells were determined. The explant from the first selection tumors and that from the second selection tumors were found. to have 53 and 54 chromosome modes, respectively. Two explants derived from separate animals in the third stage of selection each showed different modes of chromosome number (53 and 54). The explants with a modal chromosome number of 54 had a more homogeneous karyotype than those with 53 chromosomes, and the former appeared to be less malignant than the latter. as judged by transplantability, growth rate of the cells in the hydrocortisone treated hamster and the isologous host and histological appearance of the tumors. A possible relationship between cytogenetic variations and biological variations in the cells is discussed.

A simple, rapid method is described for separating the serum lipoproteins into clear, discrete and reproducible bands by polyacrylamide disc gel electrophoresis. The sample is prestained with Sudan Black B in a sample gel and resolved by electrophoresis in a discontinuous pH system consisting of a sample gel, concentrating gel and separating gel. 30 young healthy males and 20 young healthy females were examined with the method. The examination of 22 young people of them showed trace amount of chylomicrons. No significant sex difference could be demonstrated in the plasma concentrations of chylomicrons, β- and pre- β- and α-lipoproteins as a whole.

There are several testing methods for the detection of Australia antigen. Among them an antibody sensitized latex particle agglutination assay (LAT) for the detection is technologically simple, sensitive, and rapid. So the detection of the antigen in the 896 specimens from hepatic diseases was carried out by LAT, IEOP, and SRID and the detection of rheumatoid factor in the LAT positives was also made by the modified method of Heller et al. The results of testing the 896 specimens from blood donors are positive in 0.8%. negative in 4.1%. false positive in 5.1%. and false negative in 0.1%. And also. it is suggested that false positive to LAT is partially due to rheumatoid factor. since 7 specimens of 9 positives for rheumatoid factor were to LAT (a rate of 67.8%). and partially due to the freshness of blood, since of 59 storage specimens at 4°C for a week there were different reactions in 21 specimens between storage specimens and fresh specimens. Two false negatives in the 61 specimens from hepatic diseases showed positive reactions in diluted specimens for LAT. The cause of false negative reactions to LAT is unclear but it may partly be due to the rheumatoid factor and other factors. These results led us to the conclusions that the LAT requires further development and that the LAT appears to be useful as a preliminary screening for detection of the antigen.

In the cerebral and other arteries of dogs of diffcrent ages, histochemical studies have been made on hyaluronic acid and related muccsaccharides by means of digestion procedures with Streptomyces and testicular hyaluronidases. In the cerebral arteries, hyaluronic acid is either scanty in amount or absent, but other arteries contain histochemically demonstrable amounts of this mucosaccharide which decreases, however, with advancing age of the animals. The suppressive effects of digestion with testicular hyaluronidase upon the alcianophilia (pH 1,0) of all the arteries examined indicate that varying amounts of isomeric chondroitin sulfates are present in the arterial tissues, The technique of digestion with Streptomyces hyaluronidase established previously has been found to be useful for the histochemical identification of hyaluronic acid in the tissues of dog arteries.