Histochemical Study on the Activity of
the Enzymes in Human Hepatomas
HISANDO KOBAYASHI and HISASHI TAUCHI
pg(s) 45 - 57
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Ten cases of human hepatoma obtained at surgery and autopsy were studied
enzyme-histochemically, and compared with gastric carcinoma, intrahepatic bile duct
carcinoma and prostate carcinoma. The enzymes studied were glucose-6-phosphatase,
acid phosphatase, alkaline phosphatase, succinic dehydrogenase, cytochrome oxidase,
and peroxidase.
In conventional stained sections, case 1 was grade I and II hepatocellular
carcinoma by the classification of Edmondson. Case 2 and case 3 were grade IV or
more malignant than grade III, and the remaining were almost all grade III.
Case 1, in which all enzymes except alkaline phosphatase were histochemically
demonstrated in hepatoma cells, could belong to such minimum deviation hepatoma.
Acid phosphatase was demonstrated as more prominent granules in the hepatocellular carcinoma cells of all cases.
Succinic dehydrogenase was noticed to be very weak or negative except for
case 1, but cytochrome oxidase was positive in all cases.
Glucose-6-phosphatase was histochemically demonstrated in all cases examined,
but the activity was low except for case 1, and negative in control cancer cases.
Peroxidase was generally low in activity in all hepatomas except for case 1.
The intensity of glucose-6-phosphatase and peroxidase activities in the present
human hepatomas may be related to a more detailed and accurate differentiation of
hepatocellular carcinoma than the classification of Edmondson by conventional
stained sections.
Proliferation Kinetics of Tumor and Host
Cells after Chemotherapeutic
Treatment
AKIHIKO TAKEMITSU
pg(s) 59 - 70
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In order to find the most effective schedule of Mitomycine C (MMC) treatment
against tumor bearing individuals, an investigation was made to compare the growth
kinetics of solid and ascites tumor cells with that of intestinal mucosa cells before
and after MMC treatment. In the duodenal crypt epithelium of the mouse, desoxyribonucleic acid (DNA) synthesis as measured by thymidine uptake was first depressed
within 0.5 hours after a single injection of 40 μg/kg or 400 μg/kg of MMC, significantly increased over the control level at 24 hours and then gradually returned to
the range of the control. Similar results were obtained in mitotic activity. In
contrast, DNA synthesis in Ehrlich ascites tumor was depressed within 0.5 hours
and not recovered yet at 24 hours after the same dosage of MMC. It was also noted
that the cell repair in Yoshida solid tumor, after the damage due to MMC, was slow
as compared to that in the intestinal mucosa of the rat. These data suggest that in
the duodenal crypt epithelium some compensatory homeostatic mechanism of tissue
level, which may alter their proliferative state, may be operative after the administration of the chemotherapeutic agents. It is, therefore, recommended that antitumor
agents should be given under consideration of the different kinetics of regulatory
cell repair of host and tumor tissues.
Effect of Lumbar Sympathectomy on Muscle
Circulation in Dogs and Patients
MASAFUMI HIRAI, SEIICHI KAWAI and
SHIGEHIKO SHIONOYA
pg(s) 71 - 77
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The effect of lumbar sympathectomy on the muscle circulation was investigated
in 16 dogs with experimentally proposed ischemic limb and 23 limbs of 21 patients
with peripheral vascular diseases of the legs. Muscle blood flow was measured in the
anterior tibial muscle by using Xe-l33 clearance technique. In the experimental
situation, lumbar sympathectomy had no demonstrable effect on the rate of development of collateral circulation. In clinical study, both maximal blood flow during
exercise and post-exercise hyperemia did not significantly alterate before and after
sympathectomy.