VOLUME 30 NUMBER 3 December 1967

Current Issue

ISSN 2186-3326
(Online ISSN)
ISSN 0027-7622
(Print ISSN--v.72no.3/4)

Gas Chromatographic Analysis of Steroid Hormones
Part II. Gas Chromatographic Behaviour of Trimethylsilyl Ethers

pg(s) 269 - 307

<Abstract> - < PDF >

The potential value of trimethylsilyl ethers has been reported in the field of gas chromatographic analysis of steroids. So far as has been known, satisfactory procedures affording trimethylsilyl ethers of corticosteroids have not been attained because of variable by-products produced but such a result was by no means incomprehensible.
In the present study, an abbreviated procedure of trimethylsilylation, especially suited for use in this country, was devised and the determining conditions for conversion to the derivative was studied; required derivatives could be obtained by selecting proper conditions although satisfactory results could not be achieved for a series of naturally occurring steroids with polyfunctional groups.
The steroid number data presented here, in agreement with the original view, substantiated the regularity and the significance of the steroid numbers for various derivatives of less polar steroids. It is of interest that the degree of irregularity in steroid number was generally related to the complexity of polar groups, the presence of reactive structures in a steroid molecule; so that steroids of corticoid type, being more polar, showed steroid numbers far from the expected values. probably due to the complicated side reaction during trimethylsilylation, on the one hand, and on the other, probably due to the interrelationship between reactive structures being much more extended than so called "vicinal effect" which has been pointed out in the gas chromatographic behaviour of polyfunctional steroids.
Aspects of the Fine Structure of Light and Dark Cells in the Intrahepatic Bile Duct Epithelium of the Mouse

pg(s) 309 - 318

<Abstract> - < PDF >

Studies on the Pancreatitis in a Mouse Hepatitis Virus (MHV-3) Infection

pg(s) 319 - 325

<Abstract> - < PDF >

A Cytochemical Electron Microscopic Study of Glanulogenesis in Neutrophils Peroxidase and Dopa-Oxidase Reactions

pg(s) 327 - 340

<Abstract> - < PDF >

The present study was undertaken to demonstrate, electron microscopically, a detailed localization of peroxidase and dopa·oxidase within the neutrophils of various maturities and further to correlate them with the development of cellular organelles and with the granulogenesis.
The bone marrow of rats were used as materials. Perdxidase and dopa-oxidase reactions, as seen through an electron microscope, localize in the granules, and these two stainings show similar distributions throughout the various developing stages of the neutrophil.
However, dopa-oxidase reaction never appear in Golgi region while peroxidase reaction stains either vesicles or cisternae in neutrophilie promyelocyte and myelocyte.
These two reactions show a distinct difference in intensity and localization of stainings in the granules between the specific and nonspecific granules in neutrophil. Particularly, wtih the peroxidase reaction, a discrimination is possible between the Golgi vesicle maturing to specific grannule and that maturing to nonspecific one.
The specific granules which do not stain with these two reactions appear for the first time in metamyelocyte and increase in number in more mature neutrophils.
The Effect of Surgery on Antibody Production

pg(s) 341 - 349

<Abstract> - < PDF >

The effect of operative procedure on the antibody response produced by the injection of diphtheria toxoid has been studied on 30 Moloney-negative patients.
Impairment of antibody production was observed in patients with malignant diseases as for the result of surgery.
No clear relation was noted between the antibody response and the nutritional status.
Role of the Thymus in Thymectomized Mice

pg(s) 351 - 364

<Abstract> - < PDF >

Pulmonary Air Embolism. Reappraisal Of The Importance In Open-Heart Surgery

pg(s) 365 - 372

<Abstract> - < PDF >

Experience with the use of extracorporeal circulation and advancement in surgical technique apparently has lowered the mortality and morbidity following operation for repairing a cardiac defect.
Our early cases of open cardiac operation were carried out on beating hearts without occlusion of the pulmonary arteriy. It was customary to encounter the problem of the post-perfusion syndrome and it occasionally lead to unfortunate results. Since we have paid closer attention to the seperate occlusion of the pulmonary artery when the right heart was open, the respiratory complication and the so called post-perfusion syndrome were less often encountered.
The presented experimental study was undertaken to determine the frequency of hemodynamic as well as pathological changes on the experimentally induced pulmonary air embolism in dogs. In the course of study, increased pulmonary arterial pressure and cardiac arrhythmia were noticed immediately after the in- jection of the air into the pulmonary artery, and lesion like interstitial pneumonia was observed later.
Through the experimental and retrospective clinical study, it became clear that the prevension of the pulmonary air embolism is equally important as that of the systemic air embolism.