研究業績

2024年度

  • Tsukamoto Y, Tsukamoto N, Saiki W, Tashima Y, Furukawa JI, Kizuka Y, Narimatsu Y, Clausen H, Takeuchi H, Okajima T. Characterization of galactosyltransferase and sialyltransferase genes mediating the elongation of the extracellular O-GlcNAc glycans. Biochem Biophys Res Commun. 2024 Apr 9;703:149610. doi: 10.1016/j.bbrc.2024.149610. Epub 2024 Feb 1. PMID: 38359610.
  • Kondo Y. Innate Immune Sensing of Lysosomal Dysfunction Drives Multiple Lysosomal Storage Disorders. Trends in Glycoscience and Glycotechnology. 36 (212), E78-E78.
  • Kondo Y, Li Y, Okajima T. Efficient Escorting Strategy for Aggregation-Prone Notch EGF Repeats with Sparcl1. Molecules. 2024 Feb 27;29(5):1031. doi: 10.3390/molecules29051031. PMID: 38474544.
  • Sharma P, Zhang X, Ly K, Zhang Y, Hu Y, Ye AY, Hu J, Kim JH, Lou M, Wang C, Celuzza Q, Kondo Y, Furukawa K, Bundle DR, Furukawa K, Alt FW, Winau F. The lipid globotriaosylceramide promotes germinal center B cell responses and antiviral immunity. Science. 2024 Feb 16;383(6684):eadg0564. doi: 10.1126/science.adg0564. Epub 2024 Feb 16. PMID: 38359115.
  • Huang L*, Kondo Y*, Cao L, Han J, Li T, Zuo B, Yang F, Li Y, Ma Z, Bai X, Jiang M, Ruan C, Xia L. Novel GNE missense variants impair de novo sialylation and cause defective angiogenesis in the developing brain in mice. Blood Adv. 2024 Feb 27;8(4):991-1001. doi: 10.1182/bloodadvances.2023011490. PMID: 38237079. *Equal co-first.
  • Tashima Y, Tsukamoto Y, Tsukamoto N, Kondo Y, Uddin E, Furukawa W, Go S, Takeuchi H, Okajima T. Retention of DLK1 in the endoplasmic reticulum identifies roles for EGF domain-specific O-glycans in the secretory pathway. bioRxiv. 2024. doi: 10.1101/2024.08.31.610613.
  • Kondo Y. Two new mutations identified as possible causes of rare blood disorder. EurekAlert! https://www.eurekalert.org/news-releases/1039061, 2024.    
  • Kondo Y, Jing W, Xia L Site-1 Protease Deficiency Causes Human Skeletal Dysplasia. Trends in Glycoscience and Glycotechnology 36 (210), E17-E20, 2024.
  • ファルマシア　2024.9. 総説
    紅林佑希、塚本庸平、竹内英之　O-結合型糖鎖修飾と感染症
  • Tsukamoto Y, Okajima T.
    O-GlcNAc glycans in the mammalian extracellular environment.
    Carbohydr Res. 2025 Jan 10;549:109378. doi: 10.1016/j.carres.2025.109378. Online ahead of print.PMID: 39813972
  • Suzuki S, Mashiko T, Tsukamoto Y, Oya M, Kotani Y, Okawara S, Matsumoto T, Mizue Y, Takeuchi H, Okajima T, Itoh M.
    The N-acetylglucosaminyltransferase Radical fringe contributes to defects in JAG1-dependent turnover and signaling of NOTCH3 CADASIL mutants.
    .J Biol Chem. 2024 Oct;300(10):107787. doi: 10.1016/j.jbc.2024.107787. Epub 2024 Sep 19.PMID: 39303912 Free PMC article.

2023年度

  • Kondo Y, Okajima T. Inhibitory machinery for the functional dystroglycan glycosylation. J Biochem. 2023 Apr 26;173(5):333-335. doi: 10.1093/jb/mvad003. PMID: 36760122.
  • Kakuo M, Horii T, Tonomura N, Sato R, Ogawa M, Okajima T, Kamemura K.
    Evidence that only EWS among the FET proteins acquires a low partitioning property for the hyperosmotic stress response by O-GlcNAc glycosylation on its low-complexity domain.
    Exp Cell Res. 2023 Mar 1;424(1):113504. doi: 10.1016/j.yexcr.2023.113504. Epub 2023 Feb 2.PMID: 36736606

2022年度

  • Shao B, Hoover C, Shi H, Kondo Y, Lee RH, Chen J, Shan X, Song J, McDaniel JM, Zhou M, McGee S, Vanhoorelbeke K, Bergmeier W, López JA, George JN, Xia. Deletion of platelet CLEC-2 decreases GPIbα-mediated integrin αIIbβ3 activation and decreases thrombosis in TTP. Blood. 2022 Apr 21;139(16):2523-2533. doi: 10.1182/blood.2021012896. PMID: 35157766.
  • Kakuo M, Horii T, Tonomura N, Sato R, Ogawa M, Okajima T, Kamemura K. Evidence that only EWS among the FET proteins acquires a low partitioning property for the hyperosmotic stress response by O-GlcNAc glycosylation on its low-complexity domain. Exp Cell Res. 2023 Mar 1;424(1):113504. doi: 10.1016/j.yexcr.2023.113504. Epub 2023 Feb 2. PMID: 36736606.
  • Tsukamoto Y, Ogawa M, Yogi K, Tashima Y, Takeuchi H, Okajima T. Glycoproteomics of NOTCH1 EGF repeat fragments overexpressed with different glycosyltransferases in HEK293T cells reveals insights into O-GlcNAcylation of NOTCH1. Glycobiology. 2022 Jun 13;32(7):616-628. doi: 10.1093/glycob/cwac015. PMID: 35290457.
  • Furukawa K, Okajima T. Galactosyltransferase I is a gene responsible for progeroid variant of Ehlers-Danlos syndrome: molecular cloning and identification of mutations. Biochim Biophys Acta. 2002 Dec 19;1573(3):377-81. doi: 10.1016/s0304-4165(02)00406-3. PMID: 12417421 Review.
  • Zhang A, Tsukamoto Y, Takeuchi H, Nishiwaki K, Tashima Y, Okajima T.
    Secretory expression of mammalian NOTCH tandem epidermal growth factor-like repeats based on increased O-glycosylation. Anal Biochem. 2022 Nov 1;656:114881. doi: 10.1016/j.ab.2022.114881. Epub 2022 Sep 5. PMID: 36067866.
  • Haltiwanger RS, Wells L, Freeze HH, Jafar-Nejad H, Okajima T, Stanley P.In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Mohnen D, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH, editors. Other Classes of Eukaryotic Glycans. Essentials of Glycobiology [Internet]. 4th edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022. Chapter 13.PMID: 35536928 Free Books & Documents. Review.
  • Wang W, Okajima T, Takeuchi H. Significant Roles of Notch O-Glycosylation in Cancer. Molecules. 2022 Mar 9;27(6):1783. doi: 10.3390/molecules27061783. PMID: 35335147. Review.
  • Furukawa K, Ohmi Y, Hamamura K, Kondo Y, Ohkawa Y, Kaneko K, Hashimoto N, Yesmin F, Bhuiyan RH, Tajima O, Furukawa K. Signaling domains of cancer-associated glycolipids. Glycoconj J. 2022 Apr;39(2):145-155. doi: 10.1007/s10719-022-10051-1. Epub 2022 Mar 22. PMID: 35315508 Review.
  • Tashima Y, Hirata T, Maeda Y, Murakami Y, Kinoshita T. Differential use of p24 family members as cargo receptors for the transport of glycosylphosphatidylinositol-anchored proteins and Wnt1. J Biochem. 2022 Jan 7;171(1):75-83. doi: 10.1093/jb/mvab108. PMID: 34647572.

2021年度

  • Saiki W, Ma C, Okajima T, Takeuchi H.
    Current Views on the Roles of O-Glycosylation in Controlling Notch-Ligand Interactions. Biomolecules. 2021 Feb 18;11(2):309. doi: 10.3390/biom11020309. PMID: 33670724. Review.
  • Ohmi Y, Nishikaze T, Kitaura Y, Ito T, Yamamoto S, Sugiyama F, Matsuyama M, Takahashi Y, Takeda A, Kawahara T, Okajima T, Furukawa K, Furukawa K.  Majority of alpha2,6-sialylated glycans in the adult mouse brain exist in O-glycans: SALSA-MS analysis for knockout mice of alpha2,6-sialyltransferase genes. Glycobiology. 2021 Jun 3;31(5):557-570. doi: 10.1093/glycob/cwaa105. PMID: 33242079.
  • Barua R, Mizuno K, Tashima Y, Ogawa M, Takeuchi H, Taguchi A, Okajima T.
    Bioinformatics and Functional Analyses Implicate Potential Roles for EOGT and L-fringe in Pancreatic Cancers. Molecules. 2021 Feb 7;26(4):882. doi: 10.3390/molecules26040882. PMID: 33562410.
  • Yesmin F, Bhuiyan RH, Ohmi Y, Yamamoto S, Kaneko K, Ohkawa Y, Zhang P, Hamamura K, Cheung NV, Kotani N, Honke K, Okajima T, Kambe M, Tajima O, Furukawa K, Furukawa K. Ganglioside GD2 Enhances the Malignant Phenotypes of Melanoma Cells by Cooperating with Integrins. Int J Mol Sci. 2021 Dec 31;23(1):423. doi: 10.3390/ijms23010423. PMID: 35008849.
  • Zhang P, Ohkawa Y, Yamamoto S, Momota H, Kato A, Kaneko K, Natsume A, Farhana Y, Ohmi Y, Okajima T, Bhuiyan RH, Wakabayashi T, Furukawa K, Furukawa K. St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity. Nagoya J Med Sci. 2021 Aug;83(3):535-549. doi: 10.18999/nagjms.83.3.535. PMID: 34552288.
  • Mishima Y, Hamamura K, Kato H, Furukawa K, Tashima Y, Okajima T, Kondo H, Sato T, Miyazawa K, Goto S, Togari A. Contribution of Glucosylceramide Synthase to the Proliferation of Mouse Osteoblasts. In Vivo. 2021 Nov-Dec;35(6):3111-3123. doi: 10.21873/invivo.12606. PMID: 34697142.
  • Wang W, Okajima T, Takeuchi H. Significant Roles of Notch O-Glycosylation in Cancer. Molecules. 2022 Mar 9;27(6):1783. doi: 10.3390/molecules27061783. PMID: 35335147.
  • Hoover C, Kondo Y, Shao B, McDaniel MJ, Lee R, McGee S, Whiteheart S, Bergmeier W, McEver RP, Xia L. Heightened activation of embryonic megakaryocytes causes aneurysms in the developing brain of mice lacking podoplanin. Blood. 2021 May 20;137(20):2756-2769. doi: 10.1182/blood.2020010310. PMID: 33619517.
  • Jiang Y, Tang Y, Hoover C, Kondo Y, Huang D, Restagno D, Shao B, Gao L, Michael McDaniel J, Zhou M, Silasi-Mansat R, McGee S, Jiang M, Bai X, Lupu F, Ruan C, Marth JD, Wu D, Han Y, Xia L. Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice.
    Cell Death Differ. 2021 Nov;28(11):3009-3021. doi: 10.1038/s41418-021-00797-w. Epub 2021 May 15. PMID: 33993195.
  • Kondo Y, Larabee JL, Gao L, Shi H, Shao B, Hoover CM, McDaniel JM, Ho Y, Silasi-Mansat R, Archer-Hartmann SA, Azadi P, Srinivasan RS, Rezaie AR, Borczuk A, Laurence JC, Lupu F, Ahamed J, McEver RP, Papin JF, Yu Z, Xia L. L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus. JCI Insight. 2021 Jul 22;6(14):e148999. doi: 10.1172/jci.insight.148999.
 

2020年度

  • Yesmin F, Bhuiyan RH, Ohmi Y, Ohkawa Y, Tajima O, Okajima T, Furukawa K, Furukawa K. Aminoglycosides are efficient reagents to induce readthrough of premature termination codon in mutant B4GALNT1 genes found in families of hereditary spastic paraplegia. J Biochem. in press
  • Alam SM, Tsukamoto Y, Ogawa M, Senoo Y, Ikeda K, Tashima Y, Hideyuki Takeuchi H and Okajima T. N-glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization. J Biol Chem. 295(25) 8560–8574
  • Urata Y, Saiki W,Tsukamoto Y, Sago H,Hibi H, Okajima T, Takeuchi H. Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking. Cells (2020) 9(5), 1220; https://doi.org/10.3390/cells9051220
  • Ogawa M, Tashima Y, Sakaguchi Y, Takeuchi H, Okajima T. Contribution of extracellular O-GlcNAc to the stability of folded epidermal growth factor-like domains and Notch1 trafficking. Biochem Biophys Res Commun. 526:184-190. (2020)
  • Servián-Morilla E, Cabrera-Serrano M, Johnson K, Pandey A, Ito A, Rivas E, Chamova T, Muelas N, Mongini T, Nafissi S, Claeys KG, Grewal RP, Takeuchi M, Hao H, Bönnemann C, Lopes Abath Neto O, Medne L, Brandsema J, Töpf A, Taneva A, Vilchez JJ, Tournev I, Haltiwanger RS, Takeuchi H, Jafar-Nejad H, Straub V, and Paradas C. POGLUT1 biallelic mutations cause myopathy with reduced satellite cells, α-dystroglycan hypoglycosylation and a distinctive radiological pattern. Acta Neuropathol.  2020 Mar; 139 (3): 565-82.
  • Urata Y and Takeuchi H.  Effects of Notch glycosylation on health and diseases. Dev Growth Differ 2020 Jan; 62 (1): 35-48.
 

2019年度

  • Ralser DJ, Takeuchi H, Fritz G, Basmanav FB, Effern M, Sivalingam S, El-Shabrawi-Caelen L, Degirmentepe EN, Kocatürk E, Singh M, Booken N, Spierings NMK, Schnabel V, Heineke A, Knuever J, Wolf S, Wehner M, Tronnier M, Leverkus M, Tantcheva-Poór I, Wenzel J, Oji V, Has C, Hölzel M, Frank J, Haltiwanger RS, and Betz RC.  Altered Notch signaling in Dowling-Degos disease: Additional mutations in POGLUT1 and further insights into disease pathogenesis.　J Invest Dermatol  2019 139: 960-964.
  • Takeuchi H.  Notch-Modifying Protein O-Glucosyltransferase 1 (POGLUT1): Specificities, Structures, and Human Disease Implications. Trends in Glycoscience and Glycotechnology 2019; 31: E49-E52.
  • Hashimoto N, Ito S, Tsuchida A, Bhuiyan RH, Okajima T, Yamamoto A, Furukawa K, Ohmi Y, Furukawa K. The ceramide moiety of disialoganglioside (GD3) is essential for GD3 recognition by the sialic acid-binding lectin SIGLEC7 on the cell surface. J Biol Chem. 294(28):10833-10845. (2019)
  • Bhuiyan RH, Ohmi Y, Ohkawa Y, Zhang P, Takano M, Hashimoto N, Okajima T, Furukawa K, Furukawa K. Loss of Enzyme Activity in Mutated B4GALNT1 Gene Products in Patients with Hereditary Spastic Paraplegia Results in Relatively Mild Neurological Disorders: Similarity with Phenotypes of B4galnt1 Knockout Mice. Neuroscience. 397:94-106.  (2019)
  • Yu H and Takeuchi H. Protein O-glucosylation: another essential role of glucose in biology. Curr Opin Struct Biol 2019 Jan; 56: 64-71.
 

2018年度

  • Takeuchi H*, Schneider M*, Williamson DB, Ito A, Takeuchi M, Handford PA, and Haltiwanger RS.  Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling.　Proc Natl Acad Sci USA 2018; 115 (36): E8395-402.
  • Ogawa M, Okajima T. Dissection and Whole Mount Staining of Retina from Neonatal Mice. Bio-101  DOI:   10.21769/BioProtoc.3034 (2018)
  • Zhang A, Sawaguchi S, Wan E, Ogawa M, Okajima T. Isolation of Murine Brain and Lung Microvascular Endothelial Cells   Bio-101  DOI:   10.21769/BioProtoc.2984 (2018)
  • Wan E, Ogawa M, Okajima T.  A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin. Bio-101 (2018) DOI:   10.21769/BioProtoc.3064
  • Ogawa M, Senoo Y, Ikeda K, Takeuchi H, Okajima T. Structural Divergence in O-GlcNAc Glycans Displayed on Epidermal Growth Factor-like Repeats of Mammalian Notch1. Molecules.;23(7). pii: E1745. (2018)
  • Takeuchi H, Wong D, Schneider M, Freeze HH, Takeuchi M, Berardinelli SJ, Ito A, Lee H, Nelson SF, and Haltiwanger RS. Variant in human POFUT1 reduces enzymatic activity and likely causes a recessive microcephaly, global developmental delay with cardiac and vascular features.　Glycobiology 2018 May; 28 (5): 276-83. (Recommended by Faculty of 1000 Prime)
  • Fujihira H, Usami K, Matsuno K, Takeuchi H, Denda-Nagai K, Furukawa JI, Shinohara Y, Takada A, Kawaoka Y, and Irimura T. A critical domain of Ebolavirus envelope glycoprotein determines glycoform and infectivity. Sci Rep 2018 Apr; 8 (1): 5495.
  • Schneider M, Kumar V, Nordstrom LU, Feng L, Takeuchi H, Hao H, Luca VC, Garcia KC, Stanley P, Wu P, and Haltiwanger RS. Inhibition of Delta-induced Notch signaling using fucose analogs. Nature Chem Biol 2018 Jan; 14 (1): 65-71.

2017年度

  • Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, and Semina EV. Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gen mutated in Peters Plus syndrome. Plos One 2017 Sep; 12 (9): e0184903.
  • Takeuchi H*, Yu H*, Hao H, Takeuchi M, Ito A, Li H, and Haltiwanger RS. O-Glycosylation modulates the stability of epidermal growth factor-like repeats and thereby regulates Notch trafficking. J Biol Chem  2017 Sep; 292 (38): 15964-73. (*: Equal contribution)
  • Sawaguchi S, Ogawa M, Okajima T.  Protocol for Notch-ligand Binding Assays Using Dynabeads. Bio-protocol 7: e2582 (2017)
  • Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P and Okajima T. O-GlcNAc on NOTCH1 EGF Repeats Regulates Ligand-Induced Notch Signaling and Vascular Development in Mammals. eLife 6: e24419. (2017)
  • Sheikh MO, Halmo SM, Patel S, Middleton D, Takeuchi H, Schafer CM, West CM, Haltiwanger RS, Avci FY, Moremen KW, and Wells L.  Rapid screening of sugar-nucleotide donor specificities of putative glycosyltransferases. Glycobiology 2017 Mar; 27 (3): 206-12.
  • Hubmacher D, Schneider M, Berardinelli SJ, Takeuchi H, Willard B, Reinhardt DP, Haltiwanger RS, and Apte SS. Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease. Sci Rep 2017 Feb; 7: 41871.

2016年度

  • Servián-Morilla E*, Takeuchi H*, Lee TV*, Clarimon J, Mavillard F, Area-Gómez E, Rivas E, Nieto-González JL, Rivero MC, Cabrera-Serrano M, Gómez-Sánchez L, Martínez-López JA, Estrada B, Márquez C, Morgado Y, Suárez-Calvet X, Pita G, Bigot A, Gallardo E, Fernández-Chacón R, Hirano M, Haltiwanger RS, Jafar-Nejad H, Paradas C. A POGLUT1 mutation causes a muscular dystrophy with reduced Notch signaling and satellite cell loss. EMBO Mol Med  2016 Nov; 8 (11): 1289-1309. (*: Equal contribution)
  • Yu H*, Takeuchi H*, Takeuchi M, Kantharia J, Haltiwanger RS#, and Li H#.  Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations. Nature Chem Biol  2016 Sep; 12 (9): 735-40. (*: Equal contribution, #: Co-corresponding author)
  • Benz BA, Nandadasa S, Takeuchi M, Grady RC, Takeuchi H, LoPilato RK, Kakuda S, Somerville RPT, Apte SS, Haltiwanger RS#, and Holdener BC#.  Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion. Dev Biol 2016 Aug; 416 (1): 111-22. (#: Co-corresponding author)
  • Kaneko K, Ohkawa Y, Hashimoto N, Ohmi Y, Kotani N, Honke K, Ogawa M, Okajima T, Furukawa K, Furukawa K. Neogenin defined as a GD3-associated molecule by enzyme-mediated activation of radical sources confers malignant properties via intra-cytoplasmic domain in melanoma cells. J Biol Chem. 291, 16630-16643 (2016)
  • Bhuiyan RH, Kondo Y, Yamaguchi T, Tokuda N, Ohkawa Y, Hashimoto N, Ohmi Y, Yamauchi Y, Furukawa K, Okajima T, Furukawa K. Expression analysis of 0-series gangliosides in human cancer cell lines with monoclonal antibodies generated using knockout mice of ganglioside synthase genes. Glycobiology. 26, 984-998. (2016)
  • Yamaguchi T, Yamauchi Y, Furukawa K, Ohmi Y, Ohkawa Y, Zhang Q, Okajima T, Furukawa K. Expression of B4GALNT1, an essential glycosyltransferase for the synthesis of complex gangliosides, suppresses BACE1 degradation and modulates APP processing.
    Sci Rep. 30:34505. (2016) 
  • Ji S, Tokizane K, Ohkawa Y, Ohmi Y, Banno R, Okajima T, Kiyama H, Furukawa K, Furukawa K. Increased a-series gangliosides positively regulate leptin/Ob receptor-mediated signals in hypothalamus of GD3 synthase-deficient mice. Biochem Biophys Res Commun. 479:453-460. (2016)
  • Nogimori K, Hori T, Kawaguchi K, Fukui T, Mii S, Nakada H, Matsumoto Y, Yamauchi Y, Takahashi M, Furukawa K, Okajima T, Yokoi K, Hasegawa Y, Furukawa K. Increased expression levels of ppGalNAc-T13 in lung cancers: Significance in the prognostic diagnosis. Int J Oncol. 49: 1369-1376. (2016) 
  • Valero-Gonzalez J*, Leonhard-Melief C*, Lira-Navarrete E, Jimenez-Oses G, Hernandez-Ruiz C, Pallares CP, Yruela I, Vasudevan D, Lostao A, Corzana F, Takeuchi H, Haltiwanger RS, and Hurtado-Gerrero R.  A proactive role of water molecules in acceptor recognition by Protein-O-fucosyltransferase 2. Nature Chem Biol 2016 Apr; 12 (4): 240-6. (*: Equal contribution) 

研究メンバーの過去の主要業績

  • Ogawa M, Sawaguchi S, Kawai T, Nadano D, Matsuda T, Yagi H, Kato K, Furukawa K, Okajima T. Impaired O-linked N-acetylglucosaminylation in the endoplasmic reticulum by mutated EGF domain-specific O-linked N-acetylglucosamine transferase found in Adams-Oliver syndrome. J Biol Chem 290:2137-2149 (2015)
  • Yu H, Takeuchi M, Kantharia J, LeBarron J, London E, Bakker H, Haltiwanger RS, Li H, and Takeuchi H.  Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nature Chem Biol  2015 Nov; 11 (11): 847-54.
  • Sakaidani Y, Nomura T, Matsuura A, Ito M, Suzuki E, Murakami K, Nadano D, Matsuda T, Furukawa K, Okajima T. O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions. Nat Commun 2:583 (2011)
  • Takeuchi H, Fernández-Valdivia RC, Caswell DS, Nita-Lazar A, Rana NA, Macnaughtan MA, Jafar-Nejad H, and Haltiwanger RS. Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase. Proc Natl Acad Sci USA  2011 Oct; 108 (40): 16600-5.
  • Ishikawa HO, Takeuchi H, Haltiwanger RS, and Irvine KD.  Four-jointed is a Golgi kinase that phosphorylates a subset of cadherin domains. Science 2008 Jul; 321 (5887): 401-4. (Recommended by Faculty of 1000)
  • Acar M*, Jafar-Nejad H*, Takeuchi H*, Rajan A, Ibrani D, Rana NA, Pan H, Haltiwanger RS, and Bellen H.  Rumi, a CAP10 domain protein, is a glycosyltransferase that modifies Notch and is required for Notch signaling. Cell 2008 Jan; 132 (2): 247-58. (*: Equal contribution, Recommended by Faculty of 1000)
  • Matsuura A, Ito M, Sakaidani Y, Kondo T, Murakami K, Furukawa K, Nadano D, Matsuda T, Okajima T. O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors. J Biol Chem 283:35486-35495 (2008)
  • Okajima T, Reddy B, Matsuda T, Irvine KD. Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling. BMC Biol 6:1 (2008)
  • Okajima T, Xu A, Lei L, Irvine KD. Chaperone activity of protein O-fucosyltransferase 1 promotes notch receptor folding. Science 307:1599-1603 (2005)
  • Okajima T, Irvine KD. Regulation of notch signaling by O-linked fucose. Cell 111:893-904 (2002)