The i-Score Designer calculates nine different siRNA designing scores of Ui-Tei ‚ Amarzguioui ‚ Hsieh ‚ Takasaki ‚ s-Biopredsi ‚ i-Score ‚ Reynolds ‚ Katoh ‚ and DSIR . The nine scores calculated by this service are identical to those published by individual authors except for s-Biopredsi: The correlation coefficient of s-Biopredsi and Biopredsi is 0.998. Among the nine different scores‚ three scores of i-Score ‚ s-Biopredsi ‚ and DSIR  are classified as the second generation algorithms that are more dependable than the others. s-Biopredsi and DSIR are based on 21-mer siRNA sequences‚ but only a 19-mer segment forming a RNA duplex is shown in the results. Note that there are no s-Biopredsi and DSIR scores at positions 1 and 2‚ where two additional nucleotides do not exist at the 5’end. The iScore Designer also calculates the following parameters:
Ichihara M‚ Murakumo Y‚ Masuda A‚ Matsuura T‚ Asai N‚ Jijiwa M‚ Ishida M‚ Shinmi J‚ Yatsuya H‚ Qiao S‚ Takahashi M‚ Ohno K. Thermodynamic instability of siRNA duplex is a prerequisite for dependable prediction of siRNA activities. Nucleic Acids Res 35:e123‚ 2007 [PMID: 17884914].
The siRNA Designer web service is made available by KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas "Systems Genomics" from the Ministry of Education‚ Culture‚ Sports‚ Science and Technology of Japan.
Feb 26 2009
Initiation of the iScore web-service program
Feb 28 2012 Ver.1.1 A bug that did not recognize line breaks at every 19 mer is fixed.