Production of Metastases by Treatment
with Carcinostatic Agents -III. Low Concentration of Carcinostatic
Agents on the Cells
TATSUHEI KONDO, HIDEHITO ICHIHASHI, YUICHI SHIZU,
YOSHIMASA MOMOI, YASURO KAWASHIMA,
HISAYOSHI YAMADA and MASATAKA ISHII
pg(s) 1 - 10
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The effects of treatment with various doses of carcinostatic agents on tumor
growth have been studied.
In animal experiments. treatment with low concentrations of nitrogen mustard
N-oxide resulted in an early death of mice bearing Ehrlich ascites carcinoma and
an increase in the number of circulating Yoshida sarcoma cells. The growth of
subcutaneously implanted Yoshida sarcoma was stimulated after contact of the
cells with low concentrations of nitrogen mustard N-oxide in vitro or intraperitoneally.
Low concentrations of Mitomycin-C and Chromomycin A3 also showed increase
of tumor growth and metastasis formation.
In vitro study showed that the proliferation of HeLa cells was stimulated
by adding low concentrations of nitrogen mustard N-oxide. Mitomycin-C, and
Chromomycin A3 into the culture media.
In clinical studies, much more cancer ceIls were released into the stomach
cavity after the administration of nitrogen mustard N-oxide, Mitomycin-C, and
Chromomycin A3.
Demonstration of Foetal Cells by Chromosome
Analysis of Maternal Blood
HIROYUKI AMO and GAKUJI KAJINO
pg(s) 11 - 16
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Chromosome analysis of peripheral blood cultured cells was carried out on 42
pregnancies to prove the Y-chromosome originated in the transplacental foetal
cells. The forecast of the foetal sex was confirmed after birth in 41 cases, that
is, 22 gravidae were supposed to be male foetus and 19 females.
The rate of agreement between the prenatal diagnosis and the postnatal sex
was 85.3%. The misinterpretation of the antenatal genetic diagnosis seems to
have been due to several cytogenetic and methodologic factors. But in the
present results, it is sufficient to indicate that the foetal cells, possibly the
lymphocytes, in the maternal circulation pass through the placenta during the
gestations. This phenomenon between mother and foetus would particularly
evoke immunological interest.
Age Changes in Internodal Length in the
Human Spinal Roots
-Nerve Teasing Study-
MASAKUNI MUKOYAMA
pg(s) 17 - 27
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Drug-Induced Dyskinesia
-An Electrophysiological Analysis of
Dyskinesia Induced by L-Dopa and
Anticholinergic Drugs-
TADAAKI MANO
pg(s) 29 - 48
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Effect of Heterologous Transplantation of
H4-II-E Rat Hepatoma Cells into the Cheek
Pouch of Hydrocortisone Treated Hamsters
HIDEHIKO MURAGISHI and RICHARD H. BOTTOMLEY
pg(s) 49 - 62
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A series of transplant·explant experiments using the H 4-II-E rat hepatoma
cell line and golden hamsters have been carried out. Tissue culture cells implanted
into the cheek pouch of hydrocortisone treated hamsters at a concentration of
106 cells per pouch produced tumors which histologically were typical hepatomas.
Cells from these tumors were placed back into tissue culture and were transferred
twice more into the cheek pouch of hydrocortisone treated hamsters and tissue
culture. At each stage of selection, chromosome counts, karyotypes and histological and biological characteristics were determined.
Cytogenetic studies revealed that the chromosome number in both tumors and
explanted cultures shifted and became more selected with each succeeding in vivo
and in vitro passage. The cells from the selected cultures had a much more
homogeneous karyotype than cells with the same number of chromosomes from
the original H 4-II-E culture. Tumors in the third selection had less hemorrhage
and necrosis than in the first and second selections and were slower growing and
showed lower transptantability than the original tumors produced by implantation
of the H 4-II-E cells.
These results indicate that transplantation of tissue culture cells into the
cheek pouch of hamsters treated with hydrocortisone results in the selection of
cells with a specific chromosomal constitution and altered transplantability and
growth rate suggesting that the chromosomal makeup may determine the biological
and immunological characteristics of the cell which allow it to grow under these
conditions.